| 產品編號 | bsm-33321M-FITC |
| 英文名稱 | Mouse Anti-Cyclin B1/FITC Conjugated antibody |
| 中文名稱 | FITC標記的周期素B1單克隆抗體 |
| 別 名 | CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 細胞生物 免疫學 細胞周期蛋白 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 7D1 |
| 交叉反應 | Human, (predicted: Mouse, Rat, ) |
| 產品應用 | Flow-Cyt=2ug/Test ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 48kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclin B1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. Function: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Subunit: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. Post-translational modifications: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. Database links: Entrez Gene: 891 Human Entrez Gene: 268697 Mouse Omim: 123836 Human SwissProt: P14635 Human SwissProt: P24860 Mouse Unigene: 23960 Human Unigene: 260114 Mouse Unigene: 380027 Mouse Unigene: 482545 Mouse Unigene: 9232 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 主要出現在G2期。Cyclin B是細胞周期調節必不可少的條件。 細胞周期素B1是細胞周期調節因子，它的異常表達將導致細胞周期發生紊亂，致使腫瘤形成. |
| 產品圖片 |
Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal mouse serum, C-0082) at 37°C for 20 min; Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (bsm-33321M-FITC) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal mouse serum, C-0082) at 37°C for 20 min; Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (bsm-33321M-FITC) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:Hela.
Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (bsm-33321M-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:U251.
Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (bsm-33321M-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Jurkat.
Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (bsm-33321M-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:A431.
Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (bsm-33321M-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
