| 產(chǎn)品編號 | bs-19596R-PE-Cy5 |
| 英文名稱1 | Rabbit Anti-Punt/PE-Cy5 Conjugated antibody |
| 中文名稱 | PE-Cy5標記的激活受體Atr抗體 |
| 別 名 | Activin A receptor 88CD; Activin receptor; Activin receptor type II; Atr; Atr88CD; AtrII; dlhC; Dorsal holes C; j5A5; Pun; Put; STK C; STKC; TGF B; TGF beta; TGF beta l; TGF beta type receptor like; Tgf r. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學 信號轉(zhuǎn)導 干細胞 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 細胞膜受體 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 59kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Punt |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Fruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog and wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promotes its cell division in the female germline. The Signaling by TGF beta related factors requires ligand induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the type I receptor is encoded by the thick veins (tkv) gene and the type II receptor is encoded by the punt (put) gene. These receptors mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta type factors. Over expression or mutation in dpp suppress germline stem cell differentiation. The Drosophila punt gene encodes a type II serine/threonine kinase TGF beta/Dpp (Decapentaplegic) receptor. Dpp actions are mediated by its receptor Punt and Saxophone. There are 5 down stream component in the dpp signaling cascade required to block the development of various organelles including salivary glands. These are Mothers against dpp (Mad), Medea (Med) and Schnurri (Shn). Punt signaling is also responsible for calcium gradient formation during D. melanogaster development. Punt gene encodes for a homolog of vertebrate type II receptor and Punt, like thick veins (Tkv) is essential for in vivo dpp dependent patterning process. No Dpp dependent signal processing is apparent in the absence of Punt ot Tkv suggesting that both receptors acts in concert to transduce Dpp signaling. Database links: Entrez Gene: 41772 Fruit fly (Drosophila melanogaster) SwissProt: Q24468 Fruit fly (Drosophila melanogaster) Unigene: 2889 Fruit fly (Drosophila melanogaster) Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
