| 產品編號 | bs-18670R-BF594 |
| 英文名稱1 | Rabbit Anti-phospho-MAPK15 (T175 + Y177)/BF594 Conjugated antibody |
| 中文名稱 | BF594標記的磷酸化絲裂原活化蛋白激酶15抗體 |
| 別 名 | MAPK15 (phospho T175 + Y177); p-MAPK15 (phospho T175 + Y177); ERK-7; ERK-8; ERK7; ERK8; Extracellular regulated kinase 8 delta; Extracellular signal regulated kinase 8; Extracellular signal-regulated kinase 7; Extracellular signal-regulated kinase 8; MAP kinase 15; MAPK 15; Mapk15; Mitogen activated protein kinase 15; Mitogen-activated protein kinase 15; MK15_HUMAN. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, ) |
| 產品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 60kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human phospho-MAPK15 (T175 + Y177) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: MAPK15 is a 544 amino acid protein that belongs to the CMGC Ser/Thr protein kinase family (MAP kinase subfamily). MAP kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. MAPK15 functions as a catalytic kinase using ATP to produce ADP and a phosphoprotein. A TXY motif, containing one threonine and one tyrosine residue, activates the MAP kinases upon phosphorylation. MAPK15 is a ubiquitously expressed protein with highest expression found in lung and kidney. Function: In vitro, phosphorylates MBP. Tissue Specificity: Widely expressed with a maximal expression in lung and kidney. Post-translational modifications: Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 225689 Human SwissProt: Q8TD08 Human Unigene: 493169 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
