| 產品編號 | bs-16380R-Bio |
| 英文名稱1 | Rabbit Anti-HA1/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的HA1蛋白抗體 |
| 別 名 | HLA HA1; HMHA1_HUMAN; HMHA1; KIAA0223; mHag HA-1; Minor histocompatibility antigen HA 1; Minor histocompatibility protein HA 1. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 免疫學 轉運蛋白 淋巴細胞 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Sheep, ) |
| 產品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 125kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HA1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Major histocompatibility complex (MHC) molecules, which include human leukocyte antigens (HLAs), form an integral part of the immune response system. They are cell-surface receptors that bind foreign peptides and present them to cytotoxic T lymphocytes (CTLs). Minor histocompatibility antigens can form an immune response upon recognition by certain T-cells when complexed with MHC molecules. HA-1 (minor histocompatibility protein HA-1), also known as HA-1, HLA-HA1 or HMHA1, is a 1,136 amino acid GTPase activator of Rho-type GTPases. Expressed in dendritic cells, epidermal Langerhans cells, hematopoietic cells, peripheral blood mononuclear cells and all leukemia and lymphoma cell lines, HA-1 is also found in various solid tissues and tumors. Highly phosphorylated, HA-1 contains one Rho-GAP domain, a single phorbol-ester/DAG-type zinc finger and is encoded by a gene located on human chromosome 19p13.3. Function: The minor histocompatibility antigen (mHags), HA-1 is a immunogenic alloantigen shown to be responsible for graft-versus-host disease (GVHD) in HLA-identical bone marrow transplantation. The antigen has two known alleles resulting in a single amino acid polymorphism. The HA-1H allele encodes histidine, whereas the HA-1R allele encodes arginine. Subunit: HA-1 forms a complex with MHC class I HLA-A*0201. Subcellular Location: Cytoplasmic Tissue Specificity: Expressed on cells of the hematopoietic lineage. Detected in dendritic cells and epidermal Langerhans cells. Expressed in peripheral blood mononuclear cells, in all leukemia/lymphoma cell lines. Detected also in some solid tumors and tissues such as cancerous and non-cancerous tissue Similarity: Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Rho-GAP domain. Database links: Entrez Gene: 23526 Human Entrez Gene: 70719 Mouse Omim: 601155 Human SwissProt: Q92619 Human SwissProt: Q3TBD2 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
