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Rabbit Anti-phospho-Cdc25B (Ser353)/Biotin Conjugated antibody (bs-5245R-Bio)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-5245R-Bio
英文名稱 Rabbit Anti-phospho-Cdc25B (Ser353)/Biotin Conjugated antibody
中文名稱 生物素標記的磷酸化細胞分裂周期蛋白25B抗體
別    名 Cdc25B (Phospho-Ser353); Cdc25B (Phospho-S353); p-Cdc25B (Ser353); p-Cdc25B (S149); Cdc 25B; Cdc25b; Cdc-25b; CDC25HU2; Cdc25m2; Cell division cycle 25 homolog B; Cell division cycle 25B; Cell division cycle 25B isoform 1; Cell division cycle 25B isoform 2; Cell division cycle 25B isoform 3; Cell division cycle 25B isoform 4; Cell division cycle 25B isoform 5; Dual specificity phosphatase Cdc25B; M phase inducer phosphatase 2; M-phase inducer phosphatase 2; MPIP2_HUMAN.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
產品類型 磷酸化抗體 
研究領域 腫瘤  細胞生物  免疫學  細胞周期蛋白  激酶和磷酸酶  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,  (predicted: Human, Rat, )
產品應用 WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 65kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008].

Function:
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Subunit:
Interacts with MAPK14 and 14-3-3 proteins.

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Post-translational modifications:
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.

Similarity:
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Database links:

Entrez Gene: 994 Human

Entrez Gene: 12531 Mouse

Entrez Gene: 171103 Rat

Omim: 116949 Human

SwissProt: P30305 Human

SwissProt: P30306 Mouse

SwissProt: P48966 Rat

Unigene: 153752 Human

Unigene: 38444 Mouse

Unigene: 11312 Rat




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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