| 產品編號 | bs-3643R-HRP |
| 英文名稱1 | Rabbit Anti-PLASTIN3/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的絲束蛋白T Plastin抗體 |
| 別 名 | Plastin 3 (T isoform); PLS3; PLS 3; PLS-3; Plastin 3; Plastin3; Plastin-3; T fimbrin. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 69kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLASTIN3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: T Plastin is a representative of an important family of actin-cross-linking and -bundling proteins that organize most of the actin networks in cells. T Plastin is associated with the actin core bundle of microvilli, stereocilia and microspikes in epithelial cells and was proposed to participate in the assembly of these structures. In mesenchymal cells, T plastin localizes to focal adhesions and the leading edge, a broad lamellar membrane extension harboring a dynamic branched actin network. Recent studies have shown that T plastin has the potential to be a Sezary cell-specific marker valuable for diagnostic and treatment of Sezary syndrome. Function: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. Subunit: Monomer. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in a variety of organs, including muscle, brain, uterus and esophagus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR Similarity: Contains 2 actin-binding domains. Contains 4 CH (calponin-homology) domains. Contains 2 EF-hand domains. Database links: Entrez Gene: 5358 Human Entrez Gene: 102866 Mouse Omim: 300131 Human SwissProt: P13797 Human SwissProt: Q99K51 Mouse Unigene: 496622 Human Unigene: 28777 Mouse Unigene: 32103 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 絲束(纖毛素)是肌動蛋白結合蛋白家族的成員,存在于細胞支架的重組中. 絲束3是來源于纖毛素蛋白家族的T淋巴細胞特異肌動蛋白成束蛋白。近年來T Plastin在用于sezary cell綜合征的研究方面較多。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
