| 產品編號 | bsm-54335R |
| 英文名稱 | Rabbit Anti-CD46 antibody |
| 中文名稱 | CD46重組兔多克隆抗體 |
| 別 名 | MCP; CD46 antigen; CD46 molecule; CD46 molecule complement regulatory protein; Complement membrane cofactor protein antibody MCP; Measles virus receptor; Membrane cofactor protein; Membrane cofactor protein precursor; MIC 10; MIC10; TLX; TRA2.10; Trophoblast leucocyte common antigen; Trophoblast lymphocyte cross reactive antigen; Antigen identified by monoclonal antibody TRA 2 10. |
| 研究領域 | 免疫學 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4C3 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:1000-5000,IHC-P=1:50-200,IHC-F=1:50-200,ICC/IF=1:50-200,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD46 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in the regulation of the complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, platelets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells. Function: May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Subcellular Location: Isoform 1: Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane; Single-pass type I membrane protein. Note=Inner acrosomal membrane of spermatozoa. Isoform 2: Secreted (Probable). Tissue Specificity: Present only in testis (at protein level). Post-translational modifications: May be O-glycosylated (By similarity). N-glycosylated. Similarity: Contains 4 Sushi (CCP/SCR) domains. SWISS: P15529 Gene ID: 4179 Database links: Entrez Gene: 17221 Mouse Entrez Gene: 4179 Human Omim: 120920 Human SwissProt: P15529 Human SwissProt: O88174 Mouse Unigene: 510402 Human Unigene: 12884 Mouse Unigene: 163242 Rat |
| 產品圖片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: Siha (Human) Cell Lysate at 30 ug
Primary: Anti-CD46 (bsm-54335R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56-65 kD
Observed band size: 56-65 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD46) Monoclonal Antibody, Unconjugated (bsm-54335R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD46) Monoclonal Antibody, Unconjugated (bsm-54335R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD46) Monoclonal Antibody, Unconjugated (bsm-54335R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
