| 產品編號 | bs-25181R |
| 英文名稱 | Rabbit Anti-RHOJ antibody |
| 中文名稱 | RHOJ蛋白抗體 |
| 別 名 | ARHJ; FLJ14445; MGC34777; Ras homolog family member J; Ras homolog gene family member J; RAS like family 7 member B; Ras like protein family member 7B; Ras-like protein family member 7B; RASL 7B; RASL7B; Rho related GTP binding protein RhoJ; Rho-related GTP-binding protein RhoJ; RHOI; Rhoj; RHOJ_HUMAN; Tc10 like GTP binding protein TCL; TC10 like Rho GTPase; Tc10-like GTP-binding protein; TC10B; TCL. |
| 研究領域 | 細胞生物 信號轉導 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RHOJ: 61-160/214 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011] Function: GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology. Subunit: Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp. Subcellular Location: Cell membrane. Similarity: Belongs to the small GTPase superfamily. Rho family. SWISS: Q9H4E5 Gene ID: 57381 Database links: Entrez Gene: 57381 Human Entrez Gene: 80837 Mouse Omim: 607653 Human SwissProt: Q9H4E5 Human SwissProt: Q9ER71 Mouse Unigene: 656339 Human Unigene: 27467 Mouse Unigene: 228606 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (RHOJ) Polyclonal Antibody, Unconjugated (bs-25181R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
