| 產品編號 | bsm-51460M |
| 英文名稱 | LC3 Mouse mAb |
| 中文名稱 | 自噬微管相關蛋白輕鏈3單克隆抗體 |
| 別 名 | MLP3A_HUMAN; Microtubule-associated proteins 1A/1B light chain 3A; MAP1LC3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A(MAP1A/MAP1B LC3 A); Microtubule-associated protein 1 light chain 3 alpha; MLP3B_HUMAN; Microtubule-associated proteins 1A/1B light chain 3B; MAP1LC3B; MAP1ALC3; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B(MAP1A/MAP1B LC3 B); Microtubule-associated protein 1 light chain 3 beta; |
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Specific References (2) | bsm-51460M has been referenced in 2 publications.
[IF=13.281] Jinjin Shi. et al. Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. 2021 Oct 20 WB ; Human.
[IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078 WB ; Mouse.
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| 研究領域 | 腫瘤 信號轉導 細胞凋亡 轉錄調節因子 細胞分化 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | L3G1 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant protein of human MAP1LC3A/B |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Subcellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes. Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Post-translational modifications: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Similarity: Belongs to the MAP1 LC3 family. SWISS: Q9GZQ8 Gene ID: 81631 Database links: Entrez Gene: 81631 Human SwissProt: Q9GZQ8 Human |
| 產品圖片 |
Sample:
Lane 1: Brain (Mouse) Tissue Lysate
Lane 2: Brain (Rat) Tissue Lysate
Lane 3: Brain (Human) Tissue Lysate
Primary:
Anti-LC3 (bsm-51460M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 13 kD
Observed band size: 17 kD
Sample:
Human Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
Primary:
Anti-LC3 (bsm-51460M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 13 kD
Observed band size: 13 kD
Sample:
Lane 1: Y79 (soluble fraction of cell extract) cell lysate
Lane 2: 293 transfected with human LC3 (whole cell extract) cell lysate
Primary:
Anti-LC3 (bsm-51460M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 13 kD
Observed band size: 18/16 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
