| 產品編號 | bs-22952R |
| 英文名稱 | Rabbit Anti-RSL1D1 antibody |
| 中文名稱 | 細胞衰老抑制基因抗體 |
| 別 名 | CATX 11; CATX-11; CATX11; Cellular senescence inhibited gene protein; L12; PBK1; CSIG; L12; PBK1; Protein PBK1; Ribosomal L1 domain-containing protein 1; RL1D1_HUMAN; Ribosomal L1 domain containing 1. |
| 研究領域 | 腫瘤 染色質和核信號 信號轉導 細胞周期蛋白 轉錄調節因子 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse RSL1D1: 311-410/452 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
RSL1D1, also known as CATX-11, PBK1, L12 or CSIG, is a 490 amino acid nuclear protein that belongs to the ribosomal protein L1P family. Expressed in placenta, RSL1D1 contains many phosphorylated amino acid residues and is encoded by a gene that maps to human chromosome 16p13.13. Chromosome 16 encodes over 900 genes in approximately 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, though through the CREBBP gene which encodes a critical CREB binding protein. Signs of Rubinstein-Taybi include mental retardation and predisposition to tumor growth and white blood cell neoplasias. Subcellular Location: Nucleus, nucleolus. Tissue Specificity: Placenta. Similarity: Belongs to the ribosomal protein L1P family. Highly divergent. SWISS: Q8BVY0 Gene ID: 66409 Database links: Entrez Gene: 26156 Human SwissProt: O76021 Human Unigene: 401842 Human CSIG可抑制細胞衰老并延長細胞壽命 ,可能通過核糖體生物合成過程或基因轉錄調節來調控細胞衰老過程. |
| 產品圖片 |
Sample:
Lane 1: Heart (Rat) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Muscle (Rat) Lysate at 40 ug
Lane 4: Muscle (Mouse) Lysate at 40 ug
Lane 5: Placenta (Rat) Lysate at 40 ug
Lane 6: Placenta (Mouse) Lysate at 40 ug
Lane 7: Cerebrum (Rat) Lysate at 40 ug
Lane 8: Cerebrum (Mouse) Lysate at 40 ug
Lane 9: Testis (Rat) Lysate at 40 ug
Lane 10: Testis (Mouse) Lysate at 40 ug
Lane 11: Uterus (Rat) Lysate at 40 ug
Lane 12: Uterus (Mouse) Lysate at 40 ug
Primary: Anti-RSL1D1 (bs-22952R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSL1D1) Polyclonal Antibody, Unconjugated (bs-22952R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
