| 產品編號 | bsk13003 |
| 英文名稱 | Rat TNF-α ELISA Kit |
| 中文名稱 | 大鼠腫瘤壞死因子α酶聯免疫試劑盒 |
| 別 名 | TNF-alpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF-a; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; Tumour Necrosis Factor Alpha; TNFSF1A. |
 |
Specific References (5) | bsk13003 has been referenced in 5 publications.
[IF=7.419] Wei Jia-Xing. et al. The pulmonary biopharmaceutics and anti-inflammatory effects after intratracheal and intravenous administration of Re-Du-Ning injection. BIOMED PHARMACOTHER. 2023 Apr;160:114335 ELISA ; Mouse.
[IF=3.471] Chen J et al. Modulation of activated astrocytes in the hypothalamus paraventricular nucleus to prevent ventricular arrhythmia complicating acute myocardial infarction. Int J Cardiol. 2020 Jan 16. ELISA ; Rat.
[IF=2.838] Li, Lu. et al. Luteolin alleviates inflammation and autophagy of hippocampus induced by cerebral ischemia/reperfusion by activating PPAR gamma in rats. BMC Complementary Medicine and Therapies. 2022 Dec;22(1):1-13 ELISA ; Rat.
[IF=2.65] Leyu Li. et al. Fufang Fanshiliu Decoction Revealed the Antidiabetic Effect through Modulating Inflammatory Response and Gut Microbiota Composition. EVID-BASED COMPL ALT. 2022 Oct 10;2022:3255401 ELISA ; Rat.
[IF=1.813] Fan Q et al. Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement. Evid Based Complement Alternat Med. 2020 Jun 9;2020:2957094. ELISA ; Rat.
|
| 種 屬 | Rat |
| 線性范圍 | 31.25 - 2,000 pg/mL |
| 應用范圍 | S/P/CC |
| 檢測限 | 15 pg/mL |
| 適用樣品基質 | cell culture supernates, serum, and plasma. |
| 保存條件 | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.). |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
