| 產(chǎn)品編號 | bs-22782R |
| 英文名稱 | PEG3 Rabbit pAb |
| 中文名稱 | PEG3蛋白抗體 |
| 別 名 | Paternally-expressed gene 3 protein; Zinc finger and SCAN domain-containing protein 24; Kruppel-type zinc finger protein; paternally expressed 3; Paternally expressed gene 3; PEG3 protein; Pw1; Zn-finger protein Pw1; PEG3_HUMAN; Paternally-expressed gene 3 protein. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 175 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PEG3: 1011-1110/1588 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. Function: Induces apoptosis in cooperation with SIAH1A. Acts as a mediator between p53/TP53 and BAX in a neuronal death pathway that is activated by DNA damage. Acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. Possesses a tumor suppressing activity in glioma cells. Subunit: Homodimer. Interacts with SIAH1A and SIAH2. Interacts with TRAF2. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Brain, glial cells, astrocytes, embryo, placenta, testis, ovary and uterus. In the placenta it is found in the layer of villous cytotrophoblast cells while in the ovary it is found in the cells of the ovarian stroma including the thecal layers around the follicles. Expression is highly repressed in glioma cell lines. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 12 C2H2-type zinc fingers. Contains 1 SCAN box domain. Gene ID: 18616 Database links: Entrez Gene: 18616 Mouse Entrez Gene: 66549 Mouse SwissProt: O54978 Mouse SwissProt: Q3URU2 Mouse SwissProt: Q61138 Mouse SwissProt: Q6GQS0 Mouse SwissProt: Q8R5N0 Mouse SwissProt: Q9QX53 Mouse Unigene: 7952 Mouse PEG3蛋白是一種轉(zhuǎn)錄因子。在胰腺、腦組織、胎盤、卵巢、睪丸等組織中表達(dá)，PEG3蛋白位于細(xì)胞核內(nèi)。PEG3也是一個(gè)凋亡促進(jìn)因子并可能參與調(diào)節(jié)腫瘤壞死因子(TNF)信號傳導(dǎo)通路。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PEG3) Polyclonal Antibody, Unconjugated (bs-22782R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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