| 產(chǎn)品編號 | bs-23826R |
| 英文名稱 | Rabbit Anti-B4GALT2 antibody |
| 中文名稱 | B4GALT2抗體 |
| 別 名 | Beta-1,4-Galactosyltransferase 2; UDP-Galactose:Beta-N-Acetylglucosamine Beta-1,4-Galactosyltransferase 2; UDP-Gal:Beta-GlcNAc Beta-1,4-Galactosyltransferase 2; Beta-1,4-GalTase 2; Beta4Gal-T2; B4Gal-T2; Beta-N-Acetylglucosaminyl-Glycolipid Beta-1,4-Galactosyltransferase 2; UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase, Polypeptide 2; UDP-Gal:BetaGlcNAc Beta 1,4-Galactosyltransferase, Polypeptide 2; UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase 2; Beta-4-GalT2; EC 2.4.1.-; B4Gal-T3; B4GT2_HUMAN; |
| 研究領域 | 細胞生物 合成與降解 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human B4GALT2: 201-300/372 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] Function: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose. Subcellular Location: Golgi apparatus Tissue Specificity: Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain. Similarity: Belongs to the glycosyltransferase 7 family. SWISS: O60909 Gene ID: 8704 Database links: Entrez Gene: 8704 Human Entrez Gene: 53418 Mouse Omim: 604013 Human SwissProt: O60909 Human SwissProt: Q9Z2Y2 Mouse Unigene: 632403 Human Unigene: 123843 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B4GALT2) Polyclonal Antibody, Unconjugated (bs-23826R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
