| 產品編號 | D-9801 |
| 英文名稱 | FITC |
| 中文名稱 | 熒光標記染料FITC/異硫氰酸熒光素酯 |
| 別 名 | Fluorescein isothiocyanate isomer I; 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; 5-FITC; FITC-I 綠色熒光素 |
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Specific References (5) | D-9801 has been referenced in 5 publications.
[IF=9.229] Seonhwa Lee. et al. Nanoparticle-Enabled Multiplexed Electrochemical Immunoassay for Detection of Surface Proteins on Extracellular Vesicles. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX FC ; Human.
[IF=8.713] Peng Yang. et al. Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes. ELIFE. 2022 Oct Other ; Other.
[IF=5.763] You Shuang. et al. Recombinant humanized collagen remodels endometrial immune microenvironment of chronic endometritis through macrophage immunomodulation. REGEN BIOMATER. 2023 Apr;:
[IF=3.119] Tan F et al. Identification and initial functional characterization of lysosomal integral membrane protein type 2 (LIMP-2) in turbot (Scophthalmus maximus L.). Dev Comp Immunol. 2019 Oct;99:103412. Other ;
[IF=0] Shen YY et al. Novel C-type lectin from razor clam Sinonovacula constricta agglutinates bacteria and erythrocytes in a Ca -dependent manner.Developmental & Comparative Immunology, 86, 9–16. other ;
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| CAS | 36877-69-7 |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 保存條件 | Store at -20℃, protect from light. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed. FITC分子式：
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
