| 產(chǎn)品編號 | bs-24004R |
| 英文名稱 | NEU2 Rabbit pAb |
| 中文名稱 | 細胞質(zhì)唾液酸酶2抗體 |
| 別 名 | Cytosolic sialidase; MGC129579; N acetyl alpha neuraminidase 2; N-acetyl-alpha-neuraminidase 2; Neu2; NEUR2_MOUSE; Neuraminidase 2; OTTHUMP00000164374; SIAL2; Sialidase 2; Sialidase-2. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Mouse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse NEU2: 101-200/380 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008] Function: Hydrolyzes sialylated compounds. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in skeletal muscle, fetal liver and embryonic carcinoma cell line NT2D1. Similarity: Belongs to the glycosyl hydrolase 33 family. Contains 2 BNR repeats. Database links: Entrez Gene: 4759 Human Entrez Gene: 23956Mouse SwissProt: Q9Y3R4 Human SwissProt: Q9JMH3 Mouse Unigene: 532681 Human
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| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEU2) Polyclonal Antibody, Unconjugated (bs-24004R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
