| 產品編號 | bsm-33118M |
| 英文名稱 | Peroxiredoxin 1 Mouse mAb |
| 中文名稱 | 過氧化還原酶1單克隆抗體 |
| 別 名 | Peroxiredoxin-1; Peroxiredoxin 1; Prdx 1; Prdx1; PRDX1_HUMAN; Hbp23; Heme binding 23 kDa protein; Macrophage 23 Kd stress protein; Macrophage 23kDa stress protein; Macrophase stress protein 22kDa; Macrophase stress protein 23 kd; MSP23; Natural killer cell enhancing factor A; Natural killer cell-enhancing factor A; Natural killer enhancing factor A; NKEF A; NKEF-A; NkefA; OSF3; Osteoblast specific factor 3; PAG; Paga; PAGB; Proliferation associated gene A; Proliferation associated protein PAG; Proliferation-associated gene protein; PrxI; Tdpx 2; Tdpx2; TDX2; Thioredoxin dependent peroxide reductase 2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; TPxA; Trx dependent peroxide reductase 2. |
| 研究領域 | 腫瘤 細胞生物 免疫學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 10F9 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.5ug/Test,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Peroxiredoxin 1 Protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]. Function: Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. Subunit: Homodimer; disulfide-linked, upon oxidation. May form heterodimers with AOP2. Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity). Subcellular Location: Cytoplasm. Melanosome. Post-translational modifications: Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. Similarity: Belongs to the AhpC/TSA family. Contains 1 thioredoxin domain. SWISS: Q06830 Gene ID: 5052 Database links: Entrez Gene: 5052 Human Entrez Gene: 18477 Mouse Omim: 176763 Human SwissProt: Q9JKY1 Chinese Hamster SwissProt: Q06830 Human SwissProt: P35700 Mouse Unigene: 180909 Human Unigene: 30929 Mouse Unigene: 2845 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Human Jurkat cell lysates
Lane 7: Human Raji cell lysates
Lane 8: Human MCF-7 cell lysates
Lane 9: Human A431 cell lysates
Lane 10: Human Hela cell lysates
Primary: Anti-Peroxiredoxin 1 (bsm-33118M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin 1) Monoclonal Antibody, Unconjugated (ascites of bsm-33118M 10F9) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Peroxiredoxin 1) polyclonal Antibody, Unconjugated (bsm-33118M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control（black line）:Hela.
Primary Antibody (green line): Mouse Anti-Peroxiredoxin 1 antibody (bsm-33118M)
Dilution:0.5ug/Test;
Secondary Antibody（white blue line）: Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
