| 產(chǎn)品編號 | bs-20116R |
| 英文名稱 | CSFV E2 Rabbit pAb |
| 中文名稱 | 豬瘟病毒E2抗體 |
| 別 名 | CSFV Polyprotein; CSFV E2 protein; CSFV E2; polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; CSFV Envelope glycoprotein E2; Classical swine fever virus E2; Classical swine fever virus Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus]. |
| 研究領(lǐng)域 | 免疫學(xué) 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | CSFV |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | CSFV E2 protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins. Function: E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane. P7 forms a leader sequence to properly orient NS2 in the membrane. Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome. Subunit: The E(rns) glycoprotein is found as a homodimer; disulfide-linked. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers. Subcellular Location: Host cell surface. Post-translational modifications: The E(rns) glycoprotein is heavily glycosylated. The viral RNA of pestiviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least eleven individual proteins. The N-terminal protease cleaves itself from the nascent polyprotein autocatalytically and thereby generates the N-terminus of the adjacent viral capsid protein C. Cleavage between E2 and p7 is partial. Similarity: Belongs to the pestivirus polyprotein family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 peptidase C53 domain. Contains 1 peptidase C74 domain. Contains 1 peptidase S31 domain. Contains 1 RdRp catalytic domain. Database links: Entrez Gene: 920146 CSFVA SwissProt: P19712 CSFVA 豬瘟(Classical Swine Fever,CSF)是由豬瘟病毒(CSFV)引起的豬急性高度接觸性傳染性疾病,是嚴重威脅養(yǎng)豬業(yè),具有重要經(jīng)濟意義的病毒性疾病之一,豬瘟可引起豬的全身組織器官彌漫性出血,小血管和毛細血管內(nèi)皮細胞發(fā)生變性、壞死。 豬瘟病毒為黃病毒科(Flaviviridae)瘟病毒屬(Pestivirus)成員,基因組大小約12.3kb,含一個大的開放閱讀框(ORF),由11 個編碼結(jié)構(gòu)蛋白與非結(jié)構(gòu)蛋白的基因組成。豬瘟病毒對血管組織具有親嗜性,病理組織學(xué)檢查時發(fā)現(xiàn),組織器官的出血斑點主要是由于小血管和毛細血管內(nèi)皮細胞發(fā)生腫脹、變性和壞死;梗死灶的發(fā)生主要是由于小動脈管內(nèi)皮細胞發(fā)生變性、壞死、剝脫后,血管內(nèi)膜粗糙,官腔內(nèi)血栓形成,導(dǎo)致官腔狹窄或閉塞所致。此外,還可見到心肌呈實質(zhì)變性,但病豬的腎臟等器官的實質(zhì)細胞沒有變性、壞死等病理變化。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant CSFV E2 protein
Primary: Anti-CSFV E2 (bs-20116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 32 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
