| 產品編號 | bs-23111R |
| 英文名稱 | Ly-6G Rabbit pAb |
| 中文名稱 | 淋巴細胞抗原6G抗體 |
| 別 名 | Gr-1; Gr1; Ly 6G; Ly-6G.1; Ly6G; lymphocyte antigen 6 complex locus G; Lymphocyte antigen 6G; LY6G_MOUSE. |
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Specific References (1) | bs-23111R has been referenced in 1 publications.
[IF=3.269] Meihua Ji. et al. Oxycodone protects cardiac microvascular endothelial cells against ischemia/reperfusion injury by binding to Sigma-1 Receptor. BIOENGINEERED. Bioengineered. 2022;13(4):9628-9644 IF ; Rat.
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| 研究領域 | 細胞生物 免疫學 細胞表面分子 淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse |
| 產品應用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Ly-6G: 4-50/134 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor (By similarity). Tissue Specificity: Expressed in bone marrow. Similarity: Contains 1 UPAR/Ly6 domain. SWISS: P35461 Gene ID: 546644 Database links: Entrez Gene: 546644 Mouse SwissProt: P35461 Mouse Ly6G屬于是Ly-6家族成員,主要表達于T細胞、單核細胞，中性白細胞等，在抑制抗腫瘤和T細胞的應答方面起著重要作用。只要定位于細胞膜、細胞漿。 |
| 產品圖片 |
Blank control: sp2/0.
Primary Antibody (green line): Rabbit Anti-Ly-6G antibody (bs-23111R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
