| 產(chǎn)品編號 | bs-20633R |
| 英文名稱 | HMGB1 Rabbit pAb |
| 中文名稱 | 高遷移率族蛋白B1抗體 |
| 別 名 | High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN. |
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Specific References (2) | bs-20633R has been referenced in 2 publications.
[IF=10.103] Jia Qi Liang. et al. miRNAs derived from milk small extracellular vesicles inhibit porcine epidemic diarrhea virus infection. ANTIVIR RES. 2023 Mar;:105579 WB ; Pig.
[IF=5.714] Gao R et al. Quasi-ultrafine particles promote cell metastasis via HMGB1-mediated cancer cell adhesion. Environ Pollut. 2019 Oct 23:113390. WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse HMGB1: 61-150/215 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Subunit: Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Subcellular Location: Nucleus. Chromosome. Similarity: Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains. Gene ID: 15289 Database links: Entrez Gene: 3146 Human Entrez Gene: 100862258 Mouse Entrez Gene: 15289 Mouse Omim: 163905 Human SwissProt: P09429 Human
近來的研究表明稱之為高遷移率族蛋白B-1（HMG-B1）的核內(nèi)結(jié)構(gòu)蛋白在核外表達(dá)時(shí)是一種有效的早期炎癥介質(zhì)。 高遷移性Ｂ１組蛋白（ＨＭＧＢ１）： 是一種核結(jié)合蛋白，在ＤＮＡ重組、修復(fù)、復(fù)制和基因轉(zhuǎn)錄中起作用。ＨＭＧＢ１也是巨噬細(xì)胞分泌的一種介質(zhì)。 此外，高遷移性Ｂ１組蛋白亦被受刺激的巨噬細(xì)胞或單核細(xì)胞主動(dòng)分泌。在這一主動(dòng)分泌過程中，HMGB1首先經(jīng)乙酰化并由核內(nèi)轉(zhuǎn)移至溶酶體內(nèi)，繼而在ATP和溶血磷脂膽堿兩種分泌信號指導(dǎo)下轉(zhuǎn)移至細(xì)胞外。由壞死細(xì)胞被動(dòng)釋放的HMGB1和炎癥細(xì)胞主動(dòng)分泌的HMGB1存在分子上的差異。胞外的HMGB1可作為細(xì)胞因子參與信號傳導(dǎo)，因?yàn)樗瓤勺R別Toll 樣受體（TLR）家族的一些成員，又能與識別晚期糖基化終末產(chǎn)物受體（RAGE）相作用。 HMGB1能啟動(dòng)炎癥反應(yīng)，包括產(chǎn)生多種細(xì)胞因子、對某些干細(xì)胞產(chǎn)生趨化作用、誘導(dǎo)血管粘附分子、削弱腸上皮細(xì)胞的功能等。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; I
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Inc
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A
This image was generously provided by Juanli Fu, at Southwest University in Chong Qing, China. 4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti- HMGB1 Polyclonal Antibody (bs-20633R) at 1:300 for 3 hours at 4°C, followed by Rhodamine-conjugat
Blank control (blue line): MCF7 (fixed with 80% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-20633R),Dilution: 1μg /10^6 cells;
Isotype Co
Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-20633R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
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