| 產品編號 | bs-8703R |
| 英文名稱 | Rabbit Anti-SLC25A11 antibody |
| 中文名稱 | 溶質載體家族25成員11抗體 |
| 別 名 | M2OM_HUMAN; Mitochondrial 2 oxoglutarate/malate carrier protein; Mitochondrial 2-oxoglutarate/malate carrier protein; OGC; OGCP; SLC20A4; Solute carrier family 20 (oxoglutarate carrier) member 4; Solute carrier family 20 member 4; Solute carrier family 25 (mitochondrial carrier oxoglutarate carrier) member 11; Solute carrier family 25 member 11. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Sheep,Cow,GuineaPig) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC25A15: 101-200/314 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The oxoglutarate/malate carrier transports 2-oxoglutarate across the inner membranes of mitochondria in an electroneutral exchange for malate or other dicarboxylic acids (summary by Iacobazzi et al., 1992 [PubMed 1457818]).[supplied by OMIM, Jan 2011] Function: Catalyzes the transport of 2-oxoglutarate across the inner mitochondrial membrane in an electroneutral exchange for malate or other dicarboxylic acids, and plays an important role in several metabolic processes, including the malate-aspartate shuttle, the oxoglutarate/isocitrate shuttle, in gluconeogenesis from lactate, and in nitrogen metabolism. Subcellular Location: Mitochondrion inner membrane. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. SWISS: Q02978 Gene ID: 8402 Database links: Entrez Gene: 8402 Human Entrez Gene: 67863 Mouse Omim: 604165 Human SwissProt: Q02978 Human SwissProt: Q9CR62 Mouse Unigene: 184877 Human Unigene: 296082 Mouse Unigene: 466994 Mouse Unigene: 853 Rat |
| 產品圖片 |
Sample:
Lane 1: Human U251 cell lysates
Lane 2: Human A549 cell lysates
Primary: Anti-SLC25A11 (bs-8703R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 32 kDa
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-SLC25A15 (bs-8703R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-SLC25A15 antibody (bs-8703R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
