| 產品編號 | bs-12753R |
| 英文名稱 | Rabbit Anti-POGZ/ZNF635 antibody |
| 中文名稱 | 鋅指蛋白635抗體 |
| 別 名 | KIAA0461; MGC71543; Pogo transposable element with ZNF domain; POGZ; POGZ_HUMAN; putative protein product of Nbla00003; SUHW5; suppressor of hairy wing homolog; Suppressor of hairy wing homolog 5; Zinc finger protein 280E; Zinc finger protein 635; ZNF280E; ZNF635; ZNF635M. |
| 研究領域 | 細胞生物 轉錄調節因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 155kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POGZ/ZNF635: 501-600/1410 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene appears to be a zinc finger protein containing a transposase domain at the C-terminus. This protein was found to interact with the transcription factor SP1 in a yeast two-hybrid system. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Aug 2010] Function: Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms. Subcellular Location: Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3. Similarity: Contains 9 C2H2-type zinc fingers. Contains 1 DDE domain. Contains 1 HTH CENPB-type DNA-binding domain. SWISS: Q7Z3K3 Gene ID: 23126 Database links: Entrez Gene: 23126 Human Entrez Gene: 229584 Mouse Omim: 614787 Human SwissProt: Q7Z3K3 Human SwissProt: Q8BZH4 Mouse Unigene: 489873 Human Unigene: 274787 Mouse Unigene: 103975 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
