| 產品編號 | bs-19767R |
| 英文名稱 | RBM22 Rabbit pAb |
| 中文名稱 | RNA結合蛋白22抗體 |
| 別 名 | Cwc2; FLJ10290; fSAP47; Functional spliceosome associated protein 47; Pre mRNA splicing factor RBM22; Pre-mRNA-splicing factor RBM22; RBM 22; rbm22; RBM22_HUMAN; RNA binding motif protein 22; RNA-binding motif protein 22; ZC3H16; Zinc finger CCCH domain containing protein 16; Zinc finger CCCH domain-containing protein 16. |
| 研究領域 | 細胞生物 轉錄調節因子 結合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RBM22: 101-300/420 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes an RNA binding protein. The encoded protein may play a role in cell division and may be involved in pre-mRNA splicing. Related pseudogenes exist on chromosomes 6, 7, 9, 13, 16, 18, and X. [provided by RefSeq, Mar 2009] Function: Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses. Subcellular Location: Nucleus. Cytoplasm. Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol. Similarity: Belongs to the SLT11 family. Contains 1 C3H1-type zinc finger. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q9NW64 Gene ID: 55696 Database links: Entrez Gene: 55696 Human Entrez Gene: 66810 Mouse Entrez Gene: 100345063 Rabbit Omim: 612430 Human SwissProt: Q9NW64 Human SwissProt: Q8BHS3 Mouse Unigene: 713564 Human Unigene: 275106 Mouse Unigene: 105130 Rat |
| 產品圖片 |
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-RBM22 (bs-19767R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM22) Polyclonal Antibody, Unconjugated (bs-19767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
