| 產品編號 | bs-19362R |
| 英文名稱 | Rabbit Anti-SACM1L antibody |
| 中文名稱 | 肌動蛋白抑制因子1抗體 |
| 別 名 | Phosphatidylinositide phosphatase SAC1; SAC1; SAC1 suppressor of actin mutations 1-like (yeast); SAC1_HUMAN; SACM1L; suppressor of actin 1; Suppressor of actin mutations 1-like protein. |
| 研究領域 | 細胞生物 信號轉導 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Zebrafish,Dog,Cat,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SACM1L: 431-530/587 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The Saccharomyces cerevisiae SAC1 gene modulates yeast actin function and alleviates the essential requirement for phosphatidylinositol transfer protein (sec14p) activity in Golgi secretory function. The SAC1 gene product (Sac1p) is an integral membrane lipid phosphatase of the endoplasmic reticulum (ER) and the Golgi complex and contains a Sac phosphatase domain (1-2). Sac1p functions in a wide range of cellular processes including inositol metabolism, actin cytoskeletal organization, endoplasmic reticulum ATP transport, phosphatidylinositol-phospha-tidylcholine transfer protein function and multiple-drug sensitivity (3). Sac1p is an important regulator of microsomal ATP transport, providing a link between inositol phospholipid signaling and ATP-dependent processes in the yeast ER (4). Defects in Sac1p relieves the requirement for Sec14p by altering phospholipid metabolism to expand the pool of diacylglycerol in the Golgi (5). Sac1p dysfunction exerts its pleiotropic effects on yeast Golgi function, the organization of the actin cytoskeleton, and the cellular requirement for inositol, through altered metabolism of inositol glycerophospholipids (6). These effects suggest the secretory and cytoskeletal activities are coordinated to achieve proper spatial regulation of secretion in S. cerevisiae (7). Function: Phosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Detected in heart, brain, lung, liver, kidney, pancreas and testis. Similarity: Contains 1 SAC domain. SWISS: Q9NTJ5 Gene ID: 22908 Database links: Entrez Gene: 101787561 Guinea pig Entrez Gene: 100065601 Horse Entrez Gene: 22908 Human Entrez Gene: 83493 Mouse Entrez Gene: 791142 Zebrafish Omim: 606569 Human SwissProt: Q9NTJ5 Human SwissProt: Q9EP69 Mouse SwissProt: A1L244 Zebrafish Unigene: 156509 Human Unigene: 273671 Mouse Unigene: 161978 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
