| 產品編號 | bs-19246R |
| 英文名稱 | NIF1 Rabbit pAb |
| 中文名稱 | 鋅指蛋白335抗體 |
| 別 名 | 1810045J01Rik; bA465L10.2; FZF-1; MGC86001; NIF-1; NIF-2; NIF2; NIF2, INCLUDED; NRC interacting factor 1; NRC-interacting factor 1; NRC-INTERACTING FACTOR; Nuclear Receptor Coactivator Interacting Factor 1; OTTHUMP00000031195; Zinc finger protein 335; Zinc finger/leucine zipper co transducer NIF1; zinc-finger/leucine-zipper co-transducer NIF1; ZNF335. |
| 研究領域 | 轉錄調節因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 145 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIF1: 591-690/1342 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. The encoded protein may function by altering local chromatin structure. [provided by RefSeq, Jul 2008] Function: Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Subcellular Location: Nuclear Tissue Specificity: Expression is restricted to non-neuronal tissues. SWISS: Q9H4Z2 Gene ID: 63925 Database links: Entrez Gene: 63925 Human Omim: 610827 Human SwissProt: Q9H4Z2 Human Unigene: 174193 Human |
| 產品圖片 |
Sample:
Spleen (Rat) Lysate at 40 ug
Primary: Anti-NIF1 (bs-19246R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 145 kD
Observed band size: 145 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
