| 產品編號 | bs-18700R |
| 英文名稱 | ZNF743 Rabbit pAb |
| 中文名稱 | 鋅指蛋白743抗體 |
| 別 名 | MBD2 Interacting Zinc Finger MIZF; DKFZp434F162; HiNF-P; HINFP; HINFP_HUMAN; Histone H4 gene specific protein HiNF P; Histone H4 transcription factor; Histone nuclear factor P; MBD2(methyl-CpG-binding protein)-interacting zinc finger protein; MBD2 interacting zinc finger 1; MBD2 interacting zinc finger protein; MBD2-interacting zinc finger protein; Methyl CpG binding protein 2 interacting zinc finger protein; Methyl-CpG-binding protein 2-interacting zinc finger protein; MIZF; ZNF 743. |
| 研究領域 | 轉錄調節因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF743: 301-400/517 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a transcription factor that interacts with methyl-CpG-binding protein-2 (MBD2), a component of the MeCP1 histone deacetylase (HDAC) complex, and plays a role in DNA methylation and transcription repression. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Aug 2011] Function: Transcriptional repressor that binds to the consensus sequence 5'-CGGACGTT-3' and to the RB1 promoter. Transcriptional activator that promotes histone H4 gene transcription at the G1/S phase transition in conjunction with NPAT. Also activates transcription of the ATM and PRKDC genes. Autoregulates its expression by associating with its own promoter. Subcellular Location: Nucleus. Associated with discrete nuclear foci. Tissue Specificity: Ubiquitous. Highly expressed in brain, heart, skeletal muscle, spleen, kidney, small intestine, placenta and liver. Post-translational modifications: Ubiquitinated. Ubiquitination may lead to proteasome-mediated degradation. Similarity: Contains 9 C2H2-type zinc fingers. SWISS: Q9BQA5 Gene ID: 25988 Database links: Entrez Gene: 25988 Human Entrez Gene: 102423 Mouse Omim: 607099 Human SwissProt: Q9BQA5 Human SwissProt: Q8K1K9 Mouse Unigene: 504091 Human Unigene: 24570 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF743) Polyclonal Antibody, Unconjugated (bs-18700R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
