| 產品編號 | bs-18538R |
| 英文名稱 | Rabbit Anti-PIP5K2B antibody |
| 中文名稱 | 磷脂酰肌醇-5-磷酸鹽-4激酶2型β抗體 |
| 別 名 | PIP5K2 beta; phosphatidylinositol 5-phosphate 4-kinase type-2 beta; phosphatidylinositol 5-phosphate 4-kinase type-2; 1 phosphatidylinositol 4 phosphate 5 kinase 2 beta; 1 phosphatidylinositol 4 phosphate kinase; 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta; Diphosphoinositide kinase 2 beta; Diphosphoinositide kinase 2-beta; diphosphoinositide kinase; EC 2.7.1.68; Phosphatidylinositol 4 phosphate 5 kinase type II beta; phosphatidylinositol 4 phosphate 5 kinase type II beta isoform a; Phosphatidylinositol-5-phosphate 4-kinase type II beta; Phosphatidylinositol-5-phosphate 4-kinase type-2 beta; PI(5)P 4-kinase type II beta; PI42B_HUMAN; PI5P4KB; Pip4k2b; PIP4KII-beta; PIP5K2B; PIP5KII beta; PIP5KIIB; PIP5KIIbeta; PTDINS(4) P 5 kinase; PtdIns(4) P 5-kinase isoform 2 beta; PtdIns(5)P-4-kinase isoform 2-beta. |
| 研究領域 | 細胞生物 信號轉導 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse,Xenopus laevis) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PIP5K2B: 101-200/416 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. [provided by RefSeq, Jul 2008] Function: Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Subcellular Location: Endoplasmic reticulum membrane. Cell membrane. Associated with the plasma membrane and the endoplasmic reticulum. Tissue Specificity: Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver. Similarity: Contains 1 PIPK domain. SWISS: P78356 Gene ID: 8396 Database links: Entrez Gene: 8396 Human Entrez Gene: 108083 Mouse Entrez Gene: 399394 Xenopus laevis Omim: 603261 Human SwissProt: P78356 Human SwissProt: Q80XI4 Mouse Unigene: 260603 Human Unigene: 39700 Mouse Unigene: 30025 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIP5K2B) Polyclonal Antibody, Unconjugated (bs-18538R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
