| 產品編號 | bs-18461R |
| 英文名稱 | ZC3H13 Rabbit pAb |
| 中文名稱 | ZC3H13蛋白抗體 |
| 別 名 | 2600010B19Rik; 3110050K21Rik; 4930570G11Rik; C87618; DKFZp434D1812; FLJ35669; KIAA0853; ZC3H13; ZC3HD_HUMAN; Zinc finger CCCH domain-containing protein 13; zinc finger CCCH type containing 13. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 鋅指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 196 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZC3H13: 1201-1300/1668 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ZC3H13 is a 1668 amino acid protein that contains one C3H1-type zinc finger. ZC3H13 is phosphorylated upon DNA damage, most likely by ATM or ATR. Two isoforms of ZC3H13 exists as a result of alternative splicing events. The gene encoding ZC3H13 maps to chromosome 13, which contains around 114 million base pairs and 400 genes. Key tumor suppressor genes on chromosome 13 include the breast cancer susceptibility gene, BRCA2, and the RB1 (retinoblastoma) gene. As with most chromosomes, polysomy of part or all of chromosome 13 is deleterious to development and decreases the odds of survival. Trisomy 13, also known as Patau syndrome, is quite deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. Subcellular Location: Contains 1 C3H1-type zinc finger. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 C3H1-type zinc finger. SWISS: Q5T200 Gene ID: 23091 Database links: Entrez Gene: 23091 Human Entrez Gene: 67302 Mouse SwissProt: Q5T200 Human Unigene: 136102 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZC3H13) Polyclonal Antibody, Unconjugated (bs-18461R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
