| 產(chǎn)品編號 | bs-17836R |
| 英文名稱 | Rabbit Anti-MRS2 antibody |
| 中文名稱 | MRS2蛋白抗體 |
| 別 名 | Gm902; HPT; Magnesium transporter MRS2 homolog, mitochondrial; MGC78523; MRS2 like protein; MRS2 like, magnesium homeostasis factor; MRS2 like, magnesium homeostasis factor (S. cerevisiae); MRS2 magnesium homeostasis factor homolog (S. cerevisiae); MRS2L; RPT |
| 研究領域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Cat,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MRS2: 201-300/443 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MRS2 is a magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix. There are 3 isoforms produced by alternative splicing. Function: Magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix. Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein (by similarity). Similarity: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family. SWISS: Q9HD23 Gene ID: 57380 Database links: Entrez Gene: 57380 Human Entrez Gene: 380836 Mouse SwissProt: Q9HD23 Human SwissProt: Q5NCE8 Mouse Unigene: 719256 Human |
| 產(chǎn)品圖片 |
Sample: Pancreas (mouse) Lysate at 40 ug
Primary: Anti- MRS2(bs-17836R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Muscle tissue lysates
Lane 4: Rat Heart tissue lysates
Primary: Anti-MRS2 (bs-17836R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRS2 ) Polyclonal Antibody, Unconjugated (bs-17836R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
