| 產(chǎn)品編號 | bs-15563R |
| 英文名稱 | IFT46 Rabbit pAb |
| 中文名稱 | 細胞纖毛內轉運同源蛋白46抗體 |
| 別 名 | C11orf2; C11orf60; IFT46; IFT46_HUMAN; Intraflagellar transport protein 46 homolog. |
| 研究領域 | 細胞生物 信號轉導 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IFT46: 101-200/304 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
IFT46 is a 304 amino acid protein that belongs to the IFT46 family. IFT46 localizes to the cilium basal body but can also be found along the length of the cilium and is a part of a complex involved in intraflagellar transport (IFT). In addition, IFT46 is involved in the bi-directional movement of particles that is required for the assembly, maintenance, and functionality of primary cilia. Furthermore, IFT46 may be involved in skeletogenesis and chondrocyte maturation. Two isoforms exist due to alternate splicing events and the gene encoding IFT46 maps to human chromosome 11. Chromosome 11 houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. Function: Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis (By similarity). Subunit: Part of the IFT complex B. Interacts with IFT57, IFT88 and DAW1 (By similarity). Subcellular Location: Cytoplasm, cytoskeleton, cilium basal body (By similarity). Cell projection, cilium (By similarity). Note=Expression is concentrated at the cilium basal body but is also detected along the length of the cilium (By similarity). Similarity: Belongs to the IFT46 family. SWISS: Q9NQC8 Gene ID: 56912 Database links: Entrez Gene: 56912 Human SwissProt: Q9NQC8 Human Unigene: 533738 Human |
| 產(chǎn)品圖片 |
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- IFT46 (bs-15563R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Sample:
Molt-4(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-IFT46 (bs-15563R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
