| 產品編號 | bs-13488R |
| 英文名稱 | Rabbit Anti-GOLPH3 antibody |
| 中文名稱 | 高爾基體磷蛋白3抗體 |
| 別 名 | Coat protein GPP34; FLJ90675; Golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3 (coat protein); Golgi phosphoprotein 3; GOLP3_HUMAN; Golph3; GPP34; MIDAS; Mitochondrial DNA absence factor. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞類型標志物 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GOLPH3: 51-150/298 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. GOLPH3L (golgi phosphoprotein 3-like), also known as GPP34R, is a 285 amino acid cytoplasmic protein that localizes to the Golgi apparatus. Belonging to the GOLPH3/VPS74 family, GOLPH3L may have a regulatory role in Golgi trafficking. GOLPH3L is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma. Function: Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Subunit: Homodimer. Interacts with VPS35. Subcellular Location: Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. Tissue Specificity: Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. Post-translational modifications: Phosphorylated. Similarity: Belongs to the GOLPH3/VPS74 family. SWISS: Q9H4A6 Gene ID: 64083 Database links: Entrez Gene: 64083 Human Entrez Gene: 66629 Mouse Omim: 612207 Human SwissProt: Q9H4A6 Human SwissProt: Q9CRA5 Mouse Unigene: 408909 Human Unigene: 250936 Mouse Unigene: 64519 Rat |
| 產品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- GOLPH3 (bs-13488R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 33 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
