| 產品編號 | bs-13362R |
| 英文名稱 | GJA10 Rabbit pAb |
| 中文名稱 | 間隙連接蛋白10抗體 |
| 別 名 | Gap junction alpha 10; Connexin-62; Cx62; CXA10_HUMAN; Gap junction alpha 10; Gap junction alpha 10 protein; Gap junction alpha-10 protein; Gap junction protein alpha 10 59kDa; GJA10; MGC50985; RP11-63K6.6; Gap junction alpha 10; Connexin 58; Connexin 59; Connexin-62; CX58; CX59; Cx62; CXA10_HUMAN; Gap junction alpha 10; Gap junction alpha 10 protein; Gap junction alpha 9 protein; Gap junction alpha-10 protein; Gap junction protein alpha 10 59kDa; Gap junction protein alpha 9 59kDa; GJA10; MGC50985. |
| 研究領域 | 心血管 細胞生物 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Sheep,Cow,Chicken) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GJA10: 151-250/543 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Connexins, such as GJA10, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008]. Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Involved in tracer coupling between horizontal cells of the retina. May play a role in the regulation of horizontal cell patterning. Subunit: A connexon is composed of a hexamer of connexins. Subcellular Location: Cell membrane. Cell junction; gap junction. Tissue Specificity: Expressed in skeletal muscle and heart. Similarity: Belongs to the connexin family. Alpha-type (group II) subfamily. Database links: UniProtKB/Swiss-Prot: Q969M2.1 Entrez Gene: 84694 Human Omim: 611924 Human SwissProt: P57773 Human SwissProt: Q969M2 Human Unigene: 334499 Human Unigene: 632402 Human
|
| 產品圖片 |
Tissue/cell: Rat skeletal muscle; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GJA10 Polyclonal Antibody, Unconjugated(bs-13362R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
