| 產品編號 | bs-7418R |
| 英文名稱 | Rabbit Anti-ZDHHC20 antibody |
| 中文名稱 | 鋅指結構蛋白酶20抗體 |
| 別 名 | DHHC-20; Probable palmitoyltransferase ZDHHC20; ZDH20_HUMAN; ZDHHC20; Zinc finger DHHC domain-containing protein 20. |
| 研究領域 | 細胞生物 通道蛋白 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Sheep,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DHHC-20: 241-365/365 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZDHHC20 (zinc finger, DHHC-type containing 20) is a 365 amino acid multi-pass membrane protein that contains one DHHC-type zinc finger and is thought to function as a palmitoyltransferase, catalyzing the transformation of palmitoyl-CoA and a cysteine-conjugated protein to an S-palmitoyl protein and free CoA. In response to DNA damage, ZDHHC20, which exists as multiple alternatively spliced isoforms, may be phosphorylated by ATM or ATR. The gene encoding ZDHHC20 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Function: Belongs to the DHHC palmitoyltransferase family. Contains 1 DHHC-type zinc finger. Subcellular Location: Membrane; Multi-pass membrane protein (Potential). Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the DHHC palmitoyltransferase family. Contains 1 DHHC-type zinc finger. SWISS: Q5W0Z9 Gene ID: 253832 Database links: Entrez Gene: 253832 Human Entrez Gene: 75965 Mouse SwissProt: Q5W0Z9 Human SwissProt: Q5Y5T1 Mouse Unigene: 564611 Human Unigene: 29044 Mouse Unigene: 22849 Rat |
| 產品圖片 |
Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- ZDHHC20 (bs-7418R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 44 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
