| 產品編號 | bs-4974R |
| 英文名稱 | Rabbit Anti-Glucose 6 Phosphate Dehydrogenase antibody |
| 中文名稱 | 葡萄糖-6磷酸脫氫酶抗體 |
| 別 名 | G6PD; G6PD_HUMAN; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; MET19; POS10; Zwf1p. 6磷酸葡萄糖脫氫酶; |
| 研究領域 | 心血管 細胞生物 信號轉導 糖尿病 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Horse) |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glucose 6 Phosphate Dehydrogenase: 351-450/515 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Defects in G6PD are the cause of chronic non-spherocytic hemolytic anemia (CNSHA) . Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity. Function: Produces pentose sugars for nucleic acid synthesis and main producer of NADPH reducing power. Subunit: Homodimer or homotetramer Tissue Specificity: Isoform Long is found in lymphoblasts, granulocytes and sperm. DISEASE: Defects in G6PD are the cause of chronic non-spherocytic hemolytic anemia (CNSHA) [MIM:305900]. Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity. Similarity: Belongs to the glucose-6-phosphate dehydrogenase family. SWISS: P11413 Gene ID: 2539 Database links: Entrez Gene: 2539 Human Entrez Gene: 14381 Mouse Entrez Gene: 443211 Sheep Omim: 305900 Human SwissProt: P11413 Human SwissProt: Q00612 Mouse Unigene: 461047 Human Unigene: 684904 Human Unigene: 27210 Mouse Unigene: 11040 Rat |
| 產品圖片 |
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- Glucose 6 Phosphate Dehydrogenase (bs-4974R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
