| 產(chǎn)品編號 | bs-11675R |
| 英文名稱 | UBXD2 Rabbit pAb |
| 中文名稱 | UBX結(jié)構(gòu)域域蛋白D2抗體 |
| 別 名 | Erasin; FLJ23318; KIAA0242; KIAA2042; UBX domain containing 1; UBX domain containing 2; UBX domain containing protein 2; UBXD 2; UBXDC 1; UBXDC1; UBXN4_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UBXD2: 201-300/508 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Erasin is an endoplasmic reticulum (ER) and nuclear envelope membrane protein. Expressed in a variety of tissues, such as brain, placenta, heart, liver, prostate, kidney, pancreas, lung and skeletal muscle, erasin contains one UBX domain and participates in the clearing of ERAD (endoplasmic reticulum-associated protein degradation) substrates. The UBX domain of erasin is responsible for mediating its direct interaction with VCP (valosin-containing protein), an AAA-ATPase molecular chaperone. In response to ER stress, erasin expression is induced. The knockdown of erasin expression leads to the inhibition of ERAD, suggesting an important function of erasin in the ERAD pathway. In addition, erasin may be involved in Alzheimer’s disease, as it is known to accumulate in neurofibrillary degenerating neurons in patients with Alzheimer’s disease. Function: UBXD2 is an integral membrane protein of the endoplasmic reticulum (ER) that binds valosin-containing protein (VCP) and promotes ER-associated protein degradation (ERAD). Immunohistochemical staining of brain tissue from patients with Alzheimer's disease and control subjects revealed that UBXD2 accumulates preferentially in neurons undergoing neurofibrillary degeneration in Alzheimer's disease. Subunit: Directly interacts with VCP. Subcellular Location: Endoplasmic reticulum membrane. Both the N- and the C-terminus face the cytosol; also found in the nucleus envelope contiguous to the ER. Tissue Specificity: Expressed in many tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Accumulates in Alzheimer disease-afflicted brains (at protein level). Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 UBX domain. SWISS: Q92575 Gene ID: 23190 Database links: Entrez Gene: 23190 Human Entrez Gene: 67812 Mouse Omim: 611216 Human SwissProt: Q92575 Human SwissProt: Q8VCH8 Mouse Unigene: 709238 Human |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-UBXD2 ? (bs-11675R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (bs-11675R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (bs-11675R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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