| 產品編號 | bs-11669R |
| 英文名稱 | Rabbit Anti-RNF146 antibody |
| 中文名稱 | 環指蛋白146抗體 |
| 別 名 | Dactylidin; dJ351K20.1; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1. |
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Specific References (2) | bs-11669R has been referenced in 2 publications.
[IF=9.685] Wu, Hao. et al. Crocetin antagonizes parthanatos in ischemic stroke via inhibiting NOX2 and preserving mitochondrial hexokinase-I. CELL DEATH DIS. 2023 Jan;14(1):1-13 IF,WB ; Human.
[IF=8.579] Zhou B et al. Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma. Theranostics
. 2020 May 17;10(14):6530-6543. WB ; Human.
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| 研究領域 | 環指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF146: 95-160/359 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF146 (RING finger protein 146), also known as Dactylidin, is a 359 amino acid protein that contains one RING-type zinc finger and one WWE domain. Via its RING-type zinc finger, RNF146 may play a role in transcriptional regulation and protein degradation events. Defects in the gene encoding RNF146 are associated with Alzheimer's disease (AD) and may lead to a higher risk of breast cancer. Two isoforms of RNF146 exist due to alternative splicing events. Function: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination. Subunit: Can form homooligomers. Interacts with PARsylated AXIN1, AXIN2, BLZF1, CASC3, HIST1H1C, IPO7, LIG3, NCL, PARP1, XRCC1, XRCC5 and XRCC6. Interacts with DDB1, DHX15, IQGAP1, LRPPRC, PARP2, PRKDC, RUVBL2, TNKS1 and TNKS2. Binding often leads to interactor ubiquitination, in the presence of the appropriate E1 and E2 enzymes, and proteasomal degradation. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding. Tissue Specificity: Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease. Post-translational modifications: Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding. DISEASE: Note=Defects in RNF146 are a cause of susceptibility to breast cancer. Similarity: Contains 1 RING-type zinc finger. Contains 1 WWE domain. SWISS: Q9NTX7 Gene ID: 81847 Database links: Entrez Gene: 81847 Human Omim: 612137 Human SwissProt: Q9NTX7 Human Unigene: 267120 Human |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
