| 產品編號 | bs-4778R |
| 英文名稱 | Rabbit Anti-CD30 antibody |
| 中文名稱 | CD30抗體 |
| 別 名 | CD 30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNR8_MOUSE; Tumor Necrosis Factor Receptor Superfamily Member 8; Tumor Necrosis Factor Receptor Superfamily Member 8. |
| 研究領域 | 腫瘤 細胞生物 細胞類型標志物 腫瘤細胞生物標志物 細胞因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Mouse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse TNFRSF: 51-150/498 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. Subunit: Interacts with TRAF1, TRAF2, TRAF3 and TRAF5. Subcellular Location: Membrane; Single-pass type I membrane protein (Probable). Tissue Specificity: Detected in thymus and in activated splenocytes. Similarity: Contains 3 TNFR-Cys repeats. SWISS: Q60846 Gene ID: 21941 Database links: Entrez Gene: 943 Human Entrez Gene: 21941 Mouse Omim: 153243 Human SwissProt: P28908 Human SwissProt: Q60846 Mouse Unigene: 1314 Human
CD30是一種單鏈糖蛋白，標記活化T和B淋巴細胞，R-S細胞和部分淋巴濾泡間區免疫母細胞。CD30是霍奇金淋巴瘤的一個特異性標記，也是大細胞間變型淋巴瘤的診斷型標志。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD30) Polyclonal Antibody, Unconjugated (bs-4778R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
