| 產品編號 | bs-11212R |
| 英文名稱 | Rabbit Anti-KLC1 antibody |
| 中文名稱 | 驅動蛋白2抗體 |
| 別 名 | Kinesin 2 60/70kDa; Kinesin light chain 1; Kinesin2; Kinesin-2; KLC 1; KLC; KLC1; KLC1_HUMAN; KNS 2; KNS 2A; KNS2; KNS2A; Medulloblastoma antigen MU MB 2.50; MGC15245. |
| 研究領域 | 神經生物學 信號轉導 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLC1: 1-100/573 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The kinesin family of motor proteins comprise at least two forms of conventional kinesin (kinesin-I). They are encoded by different genes and designated ubiquitous kinesin, which is expressed in all cells and tissues, and neuronal kinesin, which is expressed exclusively in neuronal cells. Conventional kinesin is a heterotetramer of two kinesin heavy chain subunits and two kinesin light chain subunits. While the kinesin heavy chain contains motor activity, evidence suggests that the kinesin light chain is involved in either modulation of kinesin heavy chain activity or in cargo binding. The motor protein kinesin is a heterotetramer composed of two heavy chains and two light chains. Kinesin motor activity is dependent on the presence of ATP and microtubules. Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. Subunit: Belongs to the kinesin light chain family. Contains 6 TPR repeats. Subcellular Location: Cytoplasm; cytoskeleton. Tissue Specificity: Found in a variety of tissues. Mostly abundant in brain and spine. Post-translational modifications: Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631. Similarity: Belongs to the kinesin light chain family. Contains 6 TPR repeats. SWISS: Q07866 Gene ID: 3831 Database links: Entrez Gene: 3831 Human Entrez Gene: 16593 Mouse Omim: 600025 Human SwissProt: Q07866 Human SwissProt: O88447 Mouse Unigene: 20107 Human Unigene: 657678 Human Unigene: 278357 Mouse Unigene: 106230 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human 293T cell lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human HeLa cell lysates
Primary: Anti-KLC1 (bs-11212R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
