| 產品編號 | bs-11076R |
| 英文名稱 | CNTNAP4 Rabbit pAb |
| 中文名稱 | 接觸蛋白相關蛋白4抗體 |
| 別 名 | CASPR 4; CASPR4; Cell recognition molecule Caspr4; Cell recognition protein CASPR4; CNTNAP 4; CNTP4_HUMAN; Contactin associated protein like 4; Contactin-associated protein-like 4. |
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Specific References (1) | bs-11076R has been referenced in 1 publications.
[IF=8.063] Zhang W et al. CNTNAP4 deficiency in dopaminergic neurons initiates parkinsonian phenotypes. Theranostics. 2020 Feb 10;10(7):3000-3021. ICC ; mouse.
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| 研究領域 | 神經生物學 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Dog,Horse) |
| 產品應用 | Flow-Cyt=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 143 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CNTNAP4: 151-250/1308 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Neurexins comprise a family of neuronal cell surface proteins, which include neurexin I (NRXN1), neurexin II (NRXN2), neurexin III (NRXN3) and CASPR (neurexin IV). CASPR4 (contactin associated protein-like 4), also known as CNTNAP4, is a 1,308 amino acid single-pass type I membrane protein belonging to the neurexin family. Possibly a cell adhesion protein, CASPR4 is expressed in multiple regions of the brain such as olfactory bulb, hippocampus and deep cerebellar nuclei, with highest expression found in spinal cord, substantia nigra and subthalamic nucleus. CASPR4 is expressed at moderate levels in fetal brain, with low expression in adult testis. Encoded by a gene that maps to human chromosome 16q23.1, CASPR4 contains four laminin G-like domains, a F5/8 type C domain, two EGF-like domains and a single fibrinogen C-terminal domain. Subcellular Location: Membrane. Similarity: Belongs to the neurexin family. Contains 2 EGF-like domains. Contains 1 F5/8 type C domain. Contains 1 fibrinogen C-terminal domain. Contains 4 laminin G-like domains. Gene ID: 85445 Database links:
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| 產品圖片 |
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-CNTNAP4 antibody (bs-11076R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
