| 產品編號 | bs-11074R |
| 英文名稱 | CNTN4/AXCAM Rabbit pAb |
| 中文名稱 | 軸突相關粘附分子抗體 |
| 別 名 | BIG 2; CNTN4A; AXCAM; Axonal associated cell adhesion molecule; BIG-2; Brain derived immunoglobulin superfamily protein 2; Brain-derived immunoglobulin superfamily protein 2; Cntn4; CNTN4_HUMAN; contactin 4; Contactin-4; Neural cell adhesion protein BIG 2; SCA16. |
| 研究領域 | 神經生物學 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 109 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CNTN4/AXCAM: 1-100/1026 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Contactin 4 is a 1,026 amino acid protein encoded by the human gene CNTN4. Contactin 4 belongs to the immunoglobulin superfamily and is a member of the Contactin family. Contactin 4 contains four fibronectin type-3 domains, six Ig-like C2-type domains, and has three isoforms (1,2,3). Defects in the CNTN4 gene are a cause of 3p deletion syndrome (3PDS). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and is characterized by developmental delay, growth retardation, and dysmorphic features. Contactin 4 is primarily expressed in brain tissue. Highest expression has been found to be in the cerebellum, with lowest levels found in corpus callosum, caudate nucleus, amygdala and spinal cord. Some expression is also found in testis, pancreas, thyroid, uterus, small intestine and kidney. Contactin 4 is not believed to be expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex. Function: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. Subcellular Location: Cell membrane. Secreted. Tissue Specificity: Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex. DISEASE: Note=A chromosomal aberration involving CNTN4 has been found in a boy with characteristic physical features of 3p deletion syndrome (3PDS). Translocation t(3;10)(p26;q26). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and characterized by developmental delay, growth retardation, and dysmorphic features. Similarity: Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q8IWV2 Gene ID: 152330 Database links: Entrez Gene: 152330 Human Entrez Gene: 269784 Mouse Omim: 607280 Human SwissProt: Q8IWV2 Human SwissProt: Q69Z26 Mouse Unigene: 298705 Human Unigene: 626418 Human Unigene: 321683 Mouse Unigene: 10117 Rat |
| 產品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CNTN4/AXCAM Polyclonal Antibody, Unconjugated(bs-11074R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
