| 產品編號 | bs-6478R |
| 英文名稱 | Rabbit Anti-Carboxypeptidase H antibody |
| 中文名稱 | 胰羧肽酶E抗體 |
| 別 名 | Carboxypeptidase E; Carboxypeptidase-E; Carboxypeptidase H; Carboxypeptidase H precursor; CarboxypeptidaseE; CarboxypeptidaseH; CBPE_HUMAN; CPE; Cph 1; CPH; Cph1; Enkephalin convertase; Entephalin convertase; Prohormone processing carboxypeptidase; Prohormone-processing carboxypeptidase. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 生長因子和激素 糖尿病 內分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細胞定位 | 細胞核 細胞漿 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Carboxypeptidase E: 201-300/476 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Subcellular Location: Isoform 1: Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted. Note=Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions. soform 2: Nucleus. Tissue Specificity: Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas. Similarity: Belongs to the peptidase M14 family. SWISS: P16870 Gene ID: 1363 Database links: Entrez Gene: 1363 Human Entrez Gene: 12876 Mouse Omim: 114855 Human SwissProt: P16870 Human SwissProt: Q00493 Mouse Unigene: 75360 Human Unigene: 31395 Mouse Unigene: 470333 Mouse Unigene: 7149 Rat |
| 產品圖片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- Carboxypeptidase H (bs-6478R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
