| 產品編號 | bs-9353R |
| 英文名稱 | phospho-Proteasome 20S alpha 3(Ser250) Rabbit pAb |
| 中文名稱 | 磷酸化蛋白酶體PSMα3抗體 |
| 別 名 | Proteasome 20S alpha 3(phospho S250); 20S Proteasome α3; HC8; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome(prosome macropain) subunit alpha type 3; Proteasome alpha 3 subunit; Proteasome component C8; Proteasome subunit alpha type 3; Proteasome subunit alpha type-3; Proteasome subunit C8; PSA3_HUMAN; PSC3; PSMA3. |
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Specific References (2) | bs-9353R has been referenced in 2 publications.
[IF=3.998] Yu-lin Huang. et al. Novel targets identified by integrated proteomic and phosphoproteomic analysis in spermatogenesis of swamp buffalo ( Bubalus bubalis ). Sci Rep-Uk. 2020 Sep;10(1):1-11 WB,IHC ; Bubalus bubalis.
[IF=2.823] Peng‐fei Zhang. et al. Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis). Mol Reprod Dev. 2021 Jan;88(1):15-33 IF ; Bovine.
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| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 細胞周期蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Proteasome 20S alpha 3 around the phosphorylation site of Ser250: DE(p-S)DD |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2. Subunit: The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with AURKB. Interacts with CDKN1A. Interacts with HIV-1 TAT protein. Interacts with hepatitis C virus (HCV) F protein. Interacts with Epstein-Barr virus EBNA3 proteins. Interacts with MDM2 and RB1. Interacts with the C-terminus of TBXA2R isoform 2. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the peptidase T1A family. SWISS: P25788 Gene ID: 5684 Database links: Entrez Gene: 5684 Human Entrez Gene: 19167 Mouse Omim: 176843 Human SwissProt: P25788 Human SwissProt: O70435 Mouse Unigene: 558799 Human Unigene: 296338 Mouse Unigene: 3997 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
