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Rabbit Anti-USP3  antibody (bs-4806R)  
~~~促銷(xiāo)代碼KT202411~~~
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產(chǎn)品編號(hào) bs-4806R
英文名稱 Rabbit Anti-USP3  antibody
中文名稱 去泛素化酶3抗體
別    名 Deubiquitinating enzyme 3; EC 3.1.2.15; SIH003; Ubiquitin carboxyl-terminal hydrolase 3; Ubiquitin specific peptidase 3; Ubiquitin specific processing protease 3; Ubiquitin Specific Protease 3; Ubiquitin thioesterase 3; Ubiquitin thiolesterase 3; Ubiquitin-specific-processing protease 3; UBP; UBP3_HUMAN; Usp3.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  激酶和磷酸酶  表觀遺傳學(xué)  泛素  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 59kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human USP3: 31-130/520 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP3 (ubiquitin specific peptidase 3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBP-type zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.

Function:
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.

Subunit:
Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.

Subcellular Location:
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.

Tissue Specificity:
Expressed in all tissues examined, with strongest expression in pancreas.

Similarity:
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger.

SWISS:
Q9Y6I4

Gene ID:
9960

Database links:

Entrez Gene: 9960 Human

Entrez Gene: 235441 Mouse

Entrez Gene: 363084 Rat

GenBank: NM_006537 Human

GenBank: NM_144937 Mouse

Omim: 604728 Human

SwissProt: Q9Y6I4 Human

SwissProt: Q91W36 Mouse

SwissProt: A1L1I5 Rat

Unigene: 458499 Human

Unigene: 38976 Mouse



產(chǎn)品圖片
Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti- USP3 (bs-4806R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Sample: A549(Human) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug Primary: Anti- USP3 (bs-4806R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
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