| 產品編號 | bs-8237R |
| 英文名稱 | Rabbit Anti-FRMD5 antibody |
| 中文名稱 | FRMD5蛋白抗體 |
| 別 名 | FERM domain containing 5; FERM domain-containing protein 5; FLJ41022; FRMD5; FRMD5_HUMAN; MGC14161. |
| 研究領域 | 細胞生物 免疫學 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FRMD5: 211-312/570 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
FERM domains are roughly 150 amino acids in length and are found in a number of cytoskeletal-associated proteins such as Ezrin, Radixin, Moesin and 4.1 (erythrocyte membrane protein band 4.1), where they provide a link between cytoskeletal signals and membrane dynamics. FRMD5 (FERM domain-containing protein 5) is a 570 amino acid single-pass membrane protein that contains one FERM domain and exists as two alternatively spliced isoforms. The gene encoding FRMD5 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes. Subcellular Location: Membrane; Single-pass membrane protein (Potential). Similarity: Contains 1 FERM domain. SWISS: Q7Z6J6 Gene ID: 84978 Database links: Entrez Gene: 84978 Human Entrez Gene: 228564 Mouse SwissProt: Q7Z6J6 Human SwissProt: Q6P5H6 Mouse Unigene: 578544 Human |
| 產品圖片 |
Sample:
HepG2 (human)cell Lysate at 40 ug
U937 (human)cell Lysate at 40 ug
Primary: Anti-FRMD5 (bs-8237R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 63 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
