| 產品編號 | bs-6909R |
| 英文名稱 | Rabbit Anti-BMP9 antibody |
| 中文名稱 | 骨形態發生蛋白9抗體 |
| 別 名 | BMP 9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2; GDF-2; growth differentiation factor 2; GDF2_HUMAN. |
| 研究領域 | 細胞生物 免疫學 信號轉導 生長因子和激素 細胞周期蛋白 轉錄調節因子 細胞分化 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP9: 351-429/429 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. [provided by RefSeq, Jul 2008]. Function: Could be involved in bone formation. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted. Similarity: Belongs to the TGF-beta family. SWISS: Q9UK05 Gene ID: 2658 Database links: Entrez Gene: 2658 Human Entrez Gene: 12165 Mouse Omim: 605120 Human SwissProt: Q9UK05 Human SwissProt: Q9WV56 Mouse Unigene: 218418 Rat |
| 產品圖片 |
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-BMP9 (bs-6909R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/47 kD
Observed band size: 50 kD
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-BMP9 (bs-6909R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/47 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP9) Polyclonal Antibody, Unconjugated (bs-6909R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
