| 產品編號 | bs-7846R |
| 英文名稱 | SKA1 Rabbit pAb |
| 中文名稱 | 紡錘體和著絲粒相關蛋白1抗體 |
| 別 名 | Ska1; SKA1_HUMAN; Spindle and kinetochore associated complex subunit 1; Spindle and kinetochore associated protein 1; Spindle and kinetochore-associated protein 1; Spindle and KT(kinetochore) associated 1; Spindle and KT associated 1; C18orf24; MGC10200. |
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Specific References (2) | bs-7846R has been referenced in 2 publications.
[IF=5.173] Zheng Li. et al. SKA1 is overexpressed in laryngocarcinoma and modulates cell growth via P53 signaling pathway. CELL CYCLE. 2022 Nov 17 WB, IHC ; Human.
[IF=3.642] Ming-Chuang Sun. et al. ETV5 overexpression promotes progression of esophageal squamous cell carcinoma by upregulating SKA1 and TRPV2. INT J MED SCI. 2022; 19(6): 1072–1081 WB ; Human.
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| 研究領域 | 細胞生物 免疫學 細胞周期蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SKA1: 181-255/255 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Subunit: Component of the SKA1 complex, composed of SKA1, SKA2 and SKA3. Forms a heterodimer with SKA2; the heterodimer interacting with SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers, each heterodimer interacting with a molecule of the SKA3 homodimer. The core SKA1 complex associates with microtubules and forms oligomeric assemblies. Interacts directly with microtubules, SKA2 and SKA3. Subcellular Location: Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Similarity: Belongs to the SKA1 family. SWISS: Q96BD8 Gene ID: 220134 Database links: Entrez Gene: 220134 Human Entrez Gene: 66468 Mouse SwissProt: Q96BD8 Human SwissProt: Q9CPV1 Mouse Unigene: 134726 Human Unigene: 283406 Mouse Unigene: 106906 Rat
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
