產品編號 | bs-7828R |
英文名稱 | RINT1 Rabbit pAb |
中文名稱 | 胞膜間內質網調節蛋白1抗體 |
別 名 | HsRINT 1; HsRINT1; RAD50 interacting protein 1; RINT 1; RINT1_HUMAN. |
研究領域 | 細胞生物 信號轉導 細胞周期蛋白 細胞分化 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Dog,Horse) |
產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 91 kDa |
檢測分子量 | |
細胞定位 | 細胞漿 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human RINT1: 201-300/792 |
亞 型 | |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
RINT1 is involved in the regulation of membrane traffic between the golgi and the endoplasmic reticulum. It may play a role in cell cycle checkpoint control and is essential for telomere length control. Function: Involved in regulation of membrane traffic between theGolgi and the endoplasmic reticulum. May play a role in cell cyclecheckpoint control. Essential for telomere length control. Subunit: Associated with a SNARE complex consisting of STX18,USE1L, BNIP1/SEC20L, and SEC22B. Interacts directly withBNIP1/SEC20L and ZW10. Interacts with RAD50 during late S and G2/Mphases. Interacts with RBL2, preferentially with the active,hypophosphorylated form. Subcellular Location: Cytoplasm. Endoplasmic reticulum membrane;Peripheral membrane protein. Similarity: Belongs to the RINT1 family. Contains 1 RINT1/TIP20 domain. SWISS: Q6NUQ1 Gene ID: 60561 Database links: UniProtKB/Swiss-Prot: Q6NUQ1.1 |
產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RCL) Polyclonal Antibody, Unconjugated (bs-7828R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |