| 產品編號 | bs-0297G-HRP |
| 英文名稱 | Goat Anti-Human IgG H&L antibody |
| 中文名稱 | 辣根過氧化物酶標記的羊抗人IgG H&L |
| 別 名 | Goat Anti-Human IgG H&L (HRP); Immunoglobulin G; |
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Specific References (17) | bs-0297G-HRP has been referenced in 17 publications.
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| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:2000-10000,IHC-P=1:100-500,IHC-F=1:100-1000,ELISA=1:1000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 2.0 mg/ml |
| 免 疫 原 | Native Human IgG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G, nonspecific adsorbed |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.br>
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| 產品圖片 |
Sample:
Lane 1,2: Human IgG (reduced form)
Lane 3,4: Human IgG (non reduced form)
Primary: Goat Anti-Human IgG H&L(HRP) (bs-0297G-HRP) at 1/2000 dilution
Predicted band size: 50,25,150 kD
Observed band size: 50,25,150 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
