| 產品編號 | bs-4556R |
| 英文名稱 | DLK1 Rabbit pAb |
| 中文名稱 | 穿膜蛋白DLK1抗體（C端） |
| 別 名 | Adipocyte differentiation inhibitor protein; Brevideltin; delta like 1 homolog; delta like homolog; delta like protein; delta1; DLK 1; DLK; DLK-1; Dlk1; DLK1_HUMAN; FA 1; FA1; Fetal antigen 1; pG2; Preadipocyte factor 1; Pref 1; Pref; Pref1; Pref-1; Protein delta homolog 1; Secredeltin; ZOG; delta1; PREF1. |
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Specific References (1) | bs-4556R has been referenced in 1 publications.
[IF=2.323] Mengyan Wang. et al. Analysis of the Bovine DLK1 Gene Polymorphism and Its Relation to Lipid Metabolism in Chinese Simmental. Animals-Basel. 2020 Jun;10(6):923 WB ; Bovine.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 信號轉導 干細胞 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Sheep) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from sheep DLK1: 301-381/383 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010]. Function: May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation. Subunit: Monomer. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart. Post-translational modifications: N- and O-glycosylated. Similarity: Contains 6 EGF-like domains. Database links: Entrez Gene: 641301 sheep Entrez Gene: 8788 Human Entrez Gene: 13386 Mouse Omim: 176290 Human SwissProt: P80370 Human SwissProt: Q09163 Mouse Unigene: 533717 Human Unigene: 157069 Mouse Unigene: 14547 Rat 穿膜蛋白DLK1又稱肝癌相關基因，是表皮生長因子(EGF)家族的成員之一,其結構特征為蛋白的細胞外區域擁有在結構及氨基酸序列上與表皮生長因子高度同源的重復結構.DLK1可以抑制脂肪前體細胞向脂肪細胞分化,在正常神經內分泌細胞及其腫瘤細胞中表達, DLK1在神經內分泌軸中起著調控細胞生長分化的作用,同時DLK1在胚胎肝、造血干細胞及早期肌肉組織中均有表達。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-DLK1 (bs-4556R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DLK1) Polyclonal Antibody, Unconjugated (bs-4556R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
