| 產品編號 | bs-6604R |
| 英文名稱 | Rabbit Anti-HCN1 antibody |
| 中文名稱 | 腦環核苷酸門控通道蛋白1/HCN1抗體 |
| 別 名 | BCNG-1; BCNG1; Brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC 2; HAC2; HCN1_HUMAN; Hyperpolarization activated cyclic nucleotide gated potassium channel 1; Potassium channel, voltage-gated, brain, 1; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1. |
| 研究領域 | 心血管 細胞生物 神經生物學 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 99kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BCNG1/HCN1: 301-400/890 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Hyperpolarization activated cation channels of the HCN gene family such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarisation and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations.
HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems. Function: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. Subunit: Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Predominantly expressed in brain. Highly expressed in apical dendrites of pyramidal neurons in the cortex, in the layer corresponding to the stratum lacunosum-moleculare in the hippocampus and in axons of basket cells in the cerebellum. Expressed in a subset of elongated cells in taste buds. Post-translational modifications: N-glycosylated. Similarity: Belongs to the immunoglobulin superfamily. ICAM family. ontains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: O60741 Gene ID: 348980 Database links: Entrez Gene: 348980 Human Entrez Gene: 15165 Mouse Omim: 602780 Human SwissProt: O60741 Human SwissProt: O88704 Mouse Unigene: 353176 Human Unigene: 650434 Human Unigene: 343429 Mouse Unigene: 21408 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
