| 產品編號 | bs-6574R |
| 英文名稱 | Rabbit Anti-RRBP1 antibody |
| 中文名稱 | 核糖體結合蛋白1抗體 |
| 別 名 | 180 kDa ribosome receptor homolog; DKFZp586A1420; ES/130; ES/130 related protein; ES130; FLJ36146; hES; Ribosome binding protein 1; Ribosome binding protein 1 homolog 180kDa; Ribosome binding protein 1 homolog; Ribosome receptor protein; RRBP 1; RRp; RRBP1_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 腫瘤細胞生物標志物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=3ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 152kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RRBP1: 641-740/1410 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
RRBP1 (Ribosome-binding protein 1) acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Function: Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane (By similarity). Subcellular Location: Endoplasmic reticulum membrane; Single-pass type III membrane protein. SWISS: Q9P2E9 Gene ID: 6238 Database links: Entrez Gene: 6238 Human Entrez Gene: 81910 Mouse Omim: 601418 Human SwissProt: Q9P2E9 Human SwissProt: Q99PL5 Mouse Unigene: 472213 Human Unigene: 13705 Mouse |
| 產品圖片 |
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-RRBP1 antibody (bs-6574R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
